Doctor Octopus's History
Dr. Olivia Octavius, also known as Doctor Octopus, is the secondary antagonist of Sony Pictures Animation's 23rd feature film Spider-Man: Into the Spider-Verse. She is an evil scientist working for the Kingpin.
Olivia Octavius is a scientist and the CEO of Alchemax, and used to be friends with Peter Parker's Aunt May. Olivia at some point later on became Doctor Octopus, and is one of the many supervillains that Spider-Man has fought in his ten years as a superhero. At some point prior to the film, she was recruited by the Kingpin as his scientific advisor, right-hand woman, and the head of his secret Super-Collider project.
After a battle between Spider-Man and Kingpin's henchmen which resulted in the Super-Collider exploding and Kingpin killing an injured Spider-Man in the process. However, the machine's effects had also caused other Spider-People to appear. An older, more disheveled version of Spider-Man from an alternate universe is summoned due to Spider-Man's DNA coming in contact with the Super-Collider as a result of being recklessly shoved in by Green Goblin, and he and Miles decide to team up to stop the super-collider and return the former to his universe. Going to Alchemax to create a new version of the USB drive (which had previously broken), Spider-Man leaves Miles behind while he infiltrates the building, however, Miles decides to come along upon seeing Kingpin and Tombstone enter the building. Kingpin begins to pressure Olivia into finishing the super-collider, while, at the same time, Miles and Spider-Man begin to take the information they need. She walks in on Spider-Man and realizes that he is from another universe. She examines the overweight counterpart before restraining him in a chair and discovers that staying too long out of his home dimension causes his cells to degenerate. Olivia then states she looks forward to it before she reveals herself to be "Doctor Octopus" and attacks Spider-Man. Miles steals the computer and its system unit, and Spider-Man follows him which leads to them being chased down by her and the Alchemax scientists.
Doctor Octopus is close to getting the computer and its hardware back before Spider-Gwen saves them and they escape. When Doctor Octopus returns without the hardware, Fisk is enraged at her for failing, though she insists that the appearance of more Spider-People only confirms that the super-collider will work and that he will be able to summon as many alternate versions of his family as he wants once it is working again after squishing a few spiders. Fisk begrudgingly sees her point and tells her to get the machine up and running.
Later, after Miles finds out that his uncle Aaron is really the Prowler and escapes from his apartment, the latter tracks him down and leads Kingpin and his other supervillain henchmen; Doctor Octopus, Scorpion and Tombstone, to Aunt May's house, where they and the other Spider-Men summoned by the super-collider (Spider-Man Noir, Peni Parker, and SP//DR, and Spider-Ham) start to fight for the USB drive which destroys the house.
That night, Kingpin hosts a gala ostensibly in honor of the fallen Spider-Man, even inviting his widow Mary Jane Watson to the affair, but in reality, only uses it as a distraction while his scientists finish rebuilding and reactivate the super-collider to summon an alternate version of Kingpin's family. The Spider-People (planning to have Peter B. Parker sacrifice himself to return the others to their dimensions without Miles trying to interfere) manage to sneak into the affair and make their way below Fisk Tower to destroy the super-collider, but Doctor Octopus, Tombstone, Scorpion and Kingpin's mobster henchmen ambush them (Kingpin and his goons having anticipated that they would come and foil the plot). The Spider-People are overwhelmed until Miles (in a new Spider-Man costume and now fully embracing his role as Spider-Man) uses Doctor Octopus's tentacles against her in invisibility mode. The Spider-People realize that he can now send them back but Kingpin turns up the accelerator. Doctor Octopus battles Miles, Spider-Man, and Spider-Woman but the Super Collider's effects of pulling things in causes a truck to run over Doctor Octopus away, to the Spider-heroes' horror.
It is completely unknown what happened to Doctor Octopus afterward, considering that she wasn't seen getting arrested along with Kingpin and his other henchmen at the end of the film, but it can be implied that if she survived the truck's impact, she could have escaped arrest or was likely sent to another dimension where she would eventually die, or that she was indeed killed by the impact and her corpse was sent to another dimension as well.
